Lipidomics

Mission Statement

To provide the highest quality of quantitative lipid measurements from biological samples including cells, tissue, serum, and media. The lab offers automated sample preparation and two distinct mass spectrometry platforms which afford a variety of specific services, ranging from shotgun lipidomics to isotope labeling and mathematical modeling of lipid flux parameters. Our full service lab provides data analysis and consultation services which provide users with accurate analysis of lipid metabolic parameters.

Services

Shotgun Lipidomics

This is a mass spectrometry-based approach that involves direct infusion of lipid samples into the AB SCIEX Lipidyzer Platform: a SCIEX 5500 triple-quadrupole (QQQ) with a Shimadzu auto-sampler configured for direct infusion experiments, SelexION ion mobility device, and Shimadzu LC. Backend analysis is performed using Sciex proprietary Lipidyzer software. When combined with SCIEX’s proprietary lipids standard sets, this instrument can provide accurate, quantitative measurements of over 1000 lipid species across 13 lipid classes within a biological sample.

Compositional Analysis

This assay quantitatively measures the fatty acid and cholesterol composition of a biological sample.  In Situ derivatization breaks up complex esterified lipids into constituent fatty acids and cholesterol, which can be measured by Gas Chromatography/Mass Spectrometer.  The assay is performed on an Agilent 7890B/5977A GC-MS.  The assay is compatible with stable isotope labeling.  Data analysis utilizes the mathematical modeling-based isotopic spectral analysis (ISA) method to provide estimations of flux and lipid synthesis rates, as well as calculating the contribution of carbon sources to the Acetyl-CoA pool.